Comparison of Self-Sustained Sequence-Replication Reaction Systems

The self-sustained sequence replication amplification system--move over, PCR.

Self-Replication of Mesa Patterns in Reaction-Diffusion Systems

Certain two-component reaction-diffusion systems on a finite interval are known to possess mesa (box-like) steadystate patterns in the singularly perturbed limit of small diffusivity for one of the two solution components. As the diffusivity D of the second component is decreased below some critical value Dc, with Dc = O(1), the existence of a steady-state mesa pattern is lost, triggering the o...

Spontaneous emergence of self-replication in chemical reaction systems

Self-replication is one of the central properties of life (1), and to explain life’s origins we need to explain how selfreplication arose. It is widely accepted that before DNA, life replicated through RNA molecules (2). However, it remains unclear how the building blocks of RNA, such as nucleotides, became available on the primitive Earth, and even if these building blocks were abundant, it is...

Self-sustained replication of an RNA enzyme.

An RNA enzyme that catalyzes the RNA-templated joining of RNA was converted to a format whereby two enzymes catalyze each other's synthesis from a total of four oligonucleotide substrates. These cross-replicating RNA enzymes undergo self-sustained exponential amplification in the absence of proteins or other biological materials. Amplification occurs with a doubling time of about 1 hour and can...

Self-sustained sequence replication (3SR): an isothermal transcription-based amplification system alternative to PCR.

T h e processes of DNA replication, DNA ligation, and RNA transcription have provided the basis of distinct and separate in vitro nucleic acid amplification strategies. The first described and the most widely utilized amplification protocol is the polymerase chain reaction (PCR).O,2) The PCR method consists of multiple cycles of oligonucleotide primer-directed DNA replication that are punctuate...